Biocides are considered a commonly used technique to kill bacteria. These are chemical substances intended to destroy or control any organism’s pathogenic activity biologically or chemically and based on the mechanism of action, biocides divided into oxidizing and non-oxidizing biocides. 

Oxidizing biocides attack microbes by oxidizing (electron transfer reaction) the cell structure, disrupting or preventing nutrients from passing across the cell wall. The non-oxidizing biocides interfere with the reproduction process, block the respiratory mechanism or break the cell wall. 

Oxidizing biocides include chlorine, chlorinedioxide, peracetic acide, hydrogen peroxide, sodium bromide, etc. Non-oxidizing biocides are quaternary ammonium compounds, glutaraldehyde, Bronopol, Tetrakis hydroxymethyl-phosphonium sulfate (THPS), 2,2-dibromo-3-nitrilopropionamide (DBNPA) and so on. The bacterial killing time can take hours or a day.

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